Date of Award
Spring 5-6-2023
Degree Type
Thesis
Degree Name
Master of Science - Biology
Department
Biology
First Advisor
Dr. Robert Wiggers
Second Advisor
Dr. Darrell Fry
Third Advisor
Dr. Karol Chandler-Ezell
Fourth Advisor
Dr. Matthew Kwiatkowski
Abstract
Inflammation is a complex and varied immune response which can be expressed chronically or as a temporary reaction to foreign invaders. Symptoms of inflammation include swelling, pain, soreness, and difficulty of motion. Recently, the effect of sesquiterpene lactones such as Helenalin from Arnica montana have been studied for their anti-inflammatory properties. In this study, we identified and harvested samples of Eupatorium serotinum, a plant historically used by Native Americans to treat a variety of medical illnesses. Crude extracts of the leaves, flowers, roots, and stems were prepared by ethanol extraction. The cytotoxicity of the different extracts on HEK293 cells was determined by a cell death assay and a cell viability assay. HPLC was performed on the extracts and compared with a Helenalin standard to determine the possible presence of sesquiterpene lactones isolated from E. serotinum. Finally, an inflammation assay was performed on TNF-α stimulated HEK293 cells pretreated with the extract, and the total RNA was extracted and sequenced to study the differential gene expression.
Repository Citation
Sloan, Jordy, "Characterization of Molecular Content, Cytotoxicity, and Anti-Inflammatory Activity of Extractions from Eupatorium serotinum" (2023). Electronic Theses and Dissertations. 494.
https://scholarworks.sfasu.edu/etds/494
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
