Date of Award
6-2019
Degree Type
Thesis
Degree Name
Master of Science - Biotechnology
Department
Biology
First Advisor
Rebecca D. Parr, Ph.D
Second Advisor
Odutayo Odunuga, Ph.D
Third Advisor
Josephine Taylor, Ph.D
Fourth Advisor
Donald Pratt, Ph.D
Abstract
Rotavirus (RV) can cause severe and deadly gastroenteritis in young children, infants, and immunocompromised individuals. Previous studies have shown that arachidin 3 (A3) inhibits RV replication, and that RV replication is dependent on the presence of lipids. This study investigated the alteration of lipid metabolism by A3 in RV infected HT29.f8 cells. A decrease in the RV regulation of lipid biosynthesis genes was observed with the addition of A3 using qRT-PCR. Also, immunofluorescent and histochemical staining for neutral fats, a major component of cellular lipid droplets, revealed an increased accumulation with both RV and RV+A3 when compared to no virus and A3 controls. Furthermore, a western blot time course study of perilipin 1 presented a cycling pattern of expression with slight variations between RV, RV+A3, and A3. This data implies an association between A3 inhibition of RV replication and lipid metabolism that could be developed into a RV therapeutic treatment.
Repository Citation
Wisdom, Stormey, "Arachidin 3 Modulation of Lipid Metabolism in Rotavirus Infections" (2019). Electronic Theses and Dissertations. 243.
https://scholarworks.sfasu.edu/etds/243
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Included in
Biochemistry Commons, Carbohydrates Commons, Digestive System Diseases Commons, Lipids Commons, Molecular Biology Commons, Other Immunology and Infectious Disease Commons, Virus Diseases Commons
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