Location

Pattillo Student Center, 2nd Floor

Start Date

29-4-2015 10:00 AM

End Date

29-4-2015 4:00 PM

Description

Rotavirus (RV) infections are the most common cause of severe diarrhea in infants and young children worldwide. The two licensed vaccines for RV protect children from common strains of RV, but they are less effective against new emerging RV strains. Therefore, new therapeutics to treat RV infections need to be developed. Recently, we have shown stilbenoids, trans-arachidin-1 (t-A1) and trans-arachidin-3 (t-A3), decrease progeny virus particles by one hundred fold. Likewise, western blot assays show a decrease in the amount of the viral protein NSP4 with the addition of the stilbenoids during a RV infection. This indicates an effect on viral replication. Immunoblot assays are a standard and cost effective means to analyze the effects of stilbenoids on RV infections.

Share

COinS

Tell us how this article helped you.

 
Apr 29th, 10:00 AM Apr 29th, 4:00 PM

Optimization of Fluorescent Detection of Rotavirus Protein NSP4 and a Cellular Receptor in two Cell Lines

Pattillo Student Center, 2nd Floor

Rotavirus (RV) infections are the most common cause of severe diarrhea in infants and young children worldwide. The two licensed vaccines for RV protect children from common strains of RV, but they are less effective against new emerging RV strains. Therefore, new therapeutics to treat RV infections need to be developed. Recently, we have shown stilbenoids, trans-arachidin-1 (t-A1) and trans-arachidin-3 (t-A3), decrease progeny virus particles by one hundred fold. Likewise, western blot assays show a decrease in the amount of the viral protein NSP4 with the addition of the stilbenoids during a RV infection. This indicates an effect on viral replication. Immunoblot assays are a standard and cost effective means to analyze the effects of stilbenoids on RV infections.