Start Date
13-4-2021 4:00 PM
End Date
13-4-2021 7:00 PM
Description
The enzyme human prolyl peptidase, or hPEP, has been linked to many processes within the brain, including breaking down neural peptides. A bovine milk protein, a-s1-casein (see above), has been found to inhibit hPEP in colon cancer cells. A 68 amino acid long section (bolded) of a-s1- casein containing the inhibitor region was cloned. The casein fragment expression was optimized for expression of the peptide. The purpose for optimizing expression is to have much of it on hand to utilize in further studies on its inhibitory properties on hPEP. By using this fragment of casein, one can study the structure and function of hPEP by testing different locations of the inhibitor sequence in the peptides to see how exactly it is inhibited.
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Expression and Purification of hPEP Inhibitor Peptide
The enzyme human prolyl peptidase, or hPEP, has been linked to many processes within the brain, including breaking down neural peptides. A bovine milk protein, a-s1-casein (see above), has been found to inhibit hPEP in colon cancer cells. A 68 amino acid long section (bolded) of a-s1- casein containing the inhibitor region was cloned. The casein fragment expression was optimized for expression of the peptide. The purpose for optimizing expression is to have much of it on hand to utilize in further studies on its inhibitory properties on hPEP. By using this fragment of casein, one can study the structure and function of hPEP by testing different locations of the inhibitor sequence in the peptides to see how exactly it is inhibited.
Comments
Faculty Sponsor: Beatrice Clack (Biology)