THE REGULATION OF ROTAVIRUS–INFECTED HT29.F8 AND MA104 CELLS TREATED WITH ARACHIDIN 1 OR ARACHIDIN 3

Author

Caleb M. Witcher, Stephen F Austin State UniversityFollow

Date of Award

Spring 5-13-2017

Degree Type

Thesis

Degree Name

Master of Science - Biology

Department

Biology

First Advisor

Rebecca Parr, Ph.D

Second Advisor

Beatrice Clack, Ph.D

Third Advisor

Josephine Taylor, Ph.D

Fourth Advisor

Donald Pratt, Ph.D

Abstract

Rotavirus (RV) infections cause severe life threatening diarrhea in young children and immunocompromised individuals. Several effective vaccines have been developed for young children but are not protective against all strains of RV, and there are no anti-RV therapeutics. Our laboratory has discovered a decrease in the number of infectious simian RV particles (SA114f) in human intestinal cell line, HT29.f8 cells with the addition of either of two stilbenoids, arachidin-1 (A1) or arachidin-3 (A3). This suggests effects on the host cell and RV replication. We examined the cellular effects of human RV strain (Wa) on a human intestinal cell line (HT29.f8) and an African green monkey kidney cell line (MA104) treated with/without either arachidin. Both cell lines demonstrated apoptotic characteristics that were modulated with the addition of either A1 or A3, and the size and population of the released virus particles were significantly altered. Likewise, the number of infectious virus particles released from the arachidin treated cells were significantly reduced. This data supports the RV therapeutic potential of A1 and A3.

Repository Citation

Witcher, Caleb M., "THE REGULATION OF ROTAVIRUS–INFECTED HT29.F8 AND MA104 CELLS TREATED WITH ARACHIDIN 1 OR ARACHIDIN 3" (2017). Electronic Theses and Dissertations. 98.
https://scholarworks.sfasu.edu/etds/98

Creative Commons License

This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.