Date of Award
5-2021
Degree Type
Thesis
Degree Name
Master of Science in Natural Science
Department
Chemistry and Biochemistry
First Advisor
Dr. Odutayo Odunuga
Second Advisor
Dr. Darrell Fry
Third Advisor
Dr. Michele Harris
Fourth Advisor
Dr. Josephine Taylor
Abstract
Striated Muscle UNC-45, also known as UNC-45b, is an important protein that acts as a chaperone for myosin in cardiac and skeletal muscles, binding to myosin at its C-terminal UCS domain and regulating its assembly into thick filaments and sarcomeric structures. The UCS domain contains a large loop that is believed to be the first point of interaction between myosin and UNC-45b. GATA-4 is an essential transcription factor that facilitates transcription of several genes in cardiac development, particularly alpha-heavy chain myosin in heart tissue. Recently, studies have shown that there is interaction of GATA-4 with UNC-45b and that GATA-4 binds to the UCS domain. The implications of this interaction suggests that UNC-45b may play a role in not only the folding of myosin, but in the transcription of it as well. The aim of this study was to identify potential interaction interfaces between GATA-4 and the loop of UNC-45b and determine if the interactions are specific through computational models. Computational analysis suggests that the UCS loop is the preferential binding site of GATA-4 and that van der Waals packing is the primary method of binding.
Repository Citation
Duncan, Drake Alexander, "Simulation of the Interaction Between Striated Muscle UNC-45 and Transcription Factor GATA-4" (2021). Electronic Theses and Dissertations. 371.
https://scholarworks.sfasu.edu/etds/371
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